Poly-l-lysine is a vital component in cell binding in cultures. This acid is crucial for researchers wishing to produce a specific result. Bosterbio Antibody and ELISA provides smears to get your project rolling quickly with peace of mind.
Can I induce cell binding in my culture?
Certain acids act as charge enhancers for your culture. They increase the number of sites available for binding.
Poly-L-Lysine, PLL, is one such charge enhancer. As a substance that adheres to many different materials, the acid is used to coat nearly anything on which a culture grows. Since the compound is clear, you lose almost no information.
How does Poly-l-lysine work?
Poly-l-lysine is a synthetically produced water-soluble amino acid. It is positively charged with one hydrobromide per unit of Lysine.
As a coating agent, the compound promotes cell adhesion. The acid further improves the survival of many primary cells while enhancing neurite outgrowth.
Promoting neurons in a culture
Unlike traditional cells, neurons are somewhat fickle. They require different stimuli from skin or other cells to continue to divide. Obtaining a significant sample for research can be stressful.
A poly-l-lysine coating helps promote neurite growth. Studies reported short neurites within 1 to 3 days after seeding on the treated glass. Double coatings promote significant division, while a single coating and laminin result in poorer performance.
Use in manufacturing stem cells
Outside the cells in the nervous system, mesenchymal cells also benefit from the use of PLL. These cells are notorious for their low survival rates.
Researchers found that PLL promotes vitality and proliferation. They determined that the acid is suitable for large scale production.
Can I use poly-l-lysine instead of poly-d-lysine?
You should use whichever compound is required for coating. Procedures calling for Lysine-D should use this compound. Deviating from the required materials may impair attachment within the culture.
However, researchers often report no significant difference in the viability of primary cell survival and neurite growth when using either Lysine-D or Lysine-L. In studies of neurons, the toxicity of your plates is often a more critical factor.
How does poly-l-lysine compare to poly-acrylic acid and poly-ethylene glycol?
A comparative study of the three compounds at the Graduate School for Applied Sciences at the University of Tsukuba in Ibaraki, Japan found that low concentrations of PLL promoted the largest amounts of adhesion, spread, proliferation, and chondrogenic differentiation.
The study also found a negative effect of using too much PLL. Poly-ethylene glycol coatings inhibited cell adhesion while enhancing cell aggregation. Poly-acrylic acid saw no significant effects. The use of any coating depends on your research needs.
Where can I find poly-l-lysine?
Bosterbio Antibody and ELISA works hard to allow researchers to hit the ground running. We are cited in over 23000 publications with our quality cultures and fast shipping. Many of our products ship the next day.
We strive to provide the quality and peace of mind that you deserve. When you need to promote proliferation, adhesion, and survival in your culture, visit our website for your PLL needs.